Forty-three night suction light trappings were conducted in a horse stable at Bet Dagan, Israel in order to determine the hourly flight activity of Culicoides imicola. Although C. imicola is active during the entire night, peak numbers of activity were found 1-3 h after sunset, which were significantly higher than the numbers caught at 4-6 h post sunset. The latter were significantly higher than those caught at 7-9 h and these were significantly higher than hours 10-12 h. During the period July – October significant differences were found between the hours 1-3 and the other hour groups, while during August – November the hour group 7-9 was significantly different from the other hour groups. No significant differences were found in the number of C. imicola at the various hour groups between summer and autumn months and no significant differences were found in the numbers of C. imicola at the various hour groups in 1996 and 2000/01 (A 119).
The method of segregating nulliparous and parous females of Culicoides spp. based on the presence of burgundy-red pigment inside the abdominal wall of parous Culicoides midges, is used worldwide. Out of 320 females of Culicoides imicola trapped by emergence traps, set over an artificial breeding site for 10 and 24 days, 73 (22.8%) showed a red pigmentation despite the fact that they were nulliparous. This finding indicated that 23% of the "parous" females that are examined for the presence of arboviruses and other pathogens or for age-grading purposes, are actually old nulliparous females, which had no chance of acquiring pathogens (A 120).